A simple, efficient and reproducible stability indicating RP-HPLC method for the simultaneous determination of esomeprazole and domperidone in pharmaceutical dosage form has been developed and validated. The separation was carried out on hypersil BDS column C18 (250 mm X 4.6 mm X 5µm) column using acetonitrile: potassium dihydrogen orthophosphate buffer (adjusted to pH 4.8 with 0.1% OPA): methanol in the ratio of 40:40:20 (v/v/v) as eluent. The flow rate was 1.0 ml/min and effluent was detected at 285 nm. The retention time of esomeprazole and domperidone were 2.959 and 3.465 min. respectively. The linear dynamic range was 20-120 ppm for esomeprazole and 15-90 ppm for domperidone, respectively. Percentage recoveries for esomeprazole and domperidone were 99.84% and 99.91% respectively. All the analytical validation parameters were determined and found in the limit as per ICH guidelines, which indicates the validity of the method. The developed stability indicating method was also found to be precise and robust for the simultaneous determination of esomeprazole and domperidone in capsule dosage forms.
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